Journal: bioRxiv
Article Title: FOXO3 regulated MIR503HG safeguards cellular quiescence by modulating PI3K/Akt pathway via miR-508/PTEN axis
doi: 10.64898/2026.03.27.714688
Figure Lengend Snippet: (A) Schematic representation of establishment of cellular quiescence induction model. (B) PI-Flow cytometry analysis of asynchronous and quiescent human diploid lung fibroblasts (WI38). (C) Quantitative representation of percentage cell population at each stage in asynchronous and quiescent WI38 cells. (D) RNA levels of MIR503HG, HES1, MXI1 and p53 examined by qRT-PCR in asynchronous, G0 and serum stimulated cells. (E) qRT-PCR analysis of MIR503HG RNA in control (scr) and MIR503HG depleted cells (MIR503HG-ASO) using antisense oligos. (F) PI-Flow cytometry analysis of WI38 cells treated with scr-oligo and MIR503HG-ASO. (G) Quantitative representation of percentage cell population at each stage in control and MIR503HG depleted cells. (H) Cell cycle profile of asynchronous HeLa cells treated with scr-oligo and MIR503HG-ASO distinguishing G0 and G1 populations using Hoechst-pyronin staining. (I) Cell cycle profile of serum starved quiescent HeLa cells treated with scr-oligo and MIR503HG-ASO distinguishing different cell populations using Hoechst-pyronin staining. (J) qRT-PCR analysis of quiescent markers HES1, MXI1 and p53 in asynchronous and quiescent cells treated with scr-oligo and MIR503HG-ASO. *: p≤0.05, **: p≤ 0.01, ***: p ≤0.001, ****: p ≤0.0001, ns: p> 0.05 by two-tailed student’s t-test, n=3. Error bars represent standard deviation.
Article Snippet: The normal human diploid lung fibroblast, WI-38 (American Type Culture Collection, Cat no-ATCC-CCL-75) was cultured in Minimum Essential Medium (MEM)(GIBCO) supplemented with 10% FBS (GIBCO), 0.1% Non-essential amino acids (MP Biomedicals), 0.1% Penicillin-Streptomycin (GIBCO) at 37°C with 95% relative humidity and 5% CO2.
Techniques: Flow Cytometry, Quantitative RT-PCR, Control, Staining, Two Tailed Test, Standard Deviation